GV - SOLAS | Moucous Membrane

38th Scientific Meeting (GV - SOLAS) Society for Laboratory Animal Science
Seminar on Isolated Perfused Organs (Essen, 2000)

	General Aspects
	Mucous Membrane
	Skin
	Lung
	Udder
	Bone
	Kidney
	Liver
	Uterus
	Intestines

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Mucous Membrane

In-vitro assay for the local compatibility in skin and mucous membranes: Testing of market products, ingredients, household- and process chemicals
Pittermann W., Förster T., B. Blume and M. Kietzmann
Henkel KGaA, D40191 Duesseldorf

There are complex interactions which may occur during the movement of molecules from the natural barrier of the outer surface of the skin into the systemic circulation [1]. The barrier properties and metabolic reactions remain comparable to living skin in this natural in vitro skin model [2]. Informations concerning irritation capability can be obtained by whole skin biopsies [3, 4, 5].

The BUS-model irritation assay allows to differentiate between the direct cytotoxicity (MTT-assay) and the reversible irritancy e.g. preinflammatory mediator concentration of PGE2 The cellular positions for both assays are located in the cell membrane (irritancy) and in the intracellulary located mitochondria (cytotoxicity).

During the last years the acute skin and mucous membrane compatibility of market products, ingredients, household- and process chemicals were tested in various study designs. Figure 1 shows the number and main exposure periods of the historical data out of this test system. The oral presentation covers the principles and results of the tests using market products, ingredients, household- and process chemicals.

Figure 1

number of studies (follicula skin)

Exposure period *
0,5 h

1,0 h

5,0 h

0,5; 1,0; 5,0 h

Open application
185

173

122

480

Occlusive application
114

124

77

315

Total
299

297

199

795

status
2000-08-04

number of studies (mucous membrane)

Total
96

2

-

*Main exposure periods (biopsies were also taken at other periods too)

[[1] D. Howes et al.; Methods for Assessing Percutaneous Absorption - The Report and Recommendations of ECVAM Workshop XIII; ATLA 24 81-106 (1996)

[2] M. Kietzmann et al.; Perfused Bovine Udder as an in Vitro Model of Percutaneous Drug Absorption. Skin Viability and Percutaneous Absorption of Dexamethasone, Benzoyl Peroxide and Etofenamate; J. Pharm.Toxicol. Meth. 30 75-84 (1993)

[3] W. Pittermann et al.; The Isolated Perfused Bovine Udder Skin Model: A New in-Vitro Model for the Assessment of Skin Penetration and Irritation; In Vitro Toxicology 10 17-21 (1997)

[4] W. Bäumer and M. Kietzmann; Effects of steroidal and nonsteroidal drugs on eicosanoid synthesis in the skin of the isolated perfused bovine udder, in Proceedings of a Workshop on Isolated Perfused Organs-Hamburg 1998 (eds.: W. Pittermann / M. Kietzmann / Ch. Grosse-Siestrup) publ. 1/2000, Lab. Animal Ldt. (London), ISBN 0-901334-12-X

[5] H. Tesmann, J. Kahre und W. Pittermann; Alkylpolyglycoside: Prüfstrategie zur Wirkungsweise an der Haut in: Nutzen und Risiko von Kosmetika; O. Braun-Falco, M. Gloor und H.C. Korting, eds.; Springer Verlag Berlin Heidelberg (2000), Seite 137-145

for review: http://www.gd-online.de/english/originals_e/Pittermann2000.htm

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August 6, 2000