38th Scientific
Meeting (GV - SOLAS)
Society for Laboratory Animal Science
(Essen, 2000)
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38th Scientific
Meeting (GV - SOLAS) |
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(Essen, 2000) |
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Udder Abstract 1 Tissue distribution of "systemically" administered antibiotics in the isolated perfused bovine udder A.M. Ehinger, B. Blume and M. Kietzmann Institute for Pharmacology, Pharmacy and Toxicology, Veterinary School Hannover, D-30559 Hannover Udders of healthy lactating cows were perfused with warmed and gassed Tyrode solution for up to 12 hours. The viability was controlled by biochemical parameters. Amoxicillin and clavulanic acid were added to the perfusion fluid to simulate a single systemic treatment. Contrary to intramammary administration, amoxicillin was equally distributed in the entire glandular tissue independent of the distance to the teat base after systemic treatment. Additionally, higher glandular concentrations of amoxicillin were measurable after impairment of the blood-udder barrier by lipopolysaccharide (LPS) treatment. Clavulanic acid generally penetrated the blood-udder barrier to a significantly lower extent. The comparision of the concentrations in the Tyrode solution with those in the perfusate showed that a significant amount of the tested compounds remained in the vasculature. top Abstract 2 The use of microdialysis technique for isolated perfused organs W. Bäumer, M. Kietzmann Institute for Pharmacology, Pharmacy and Toxicology, Veterinary School Hannover, D-30559 Hannover The microdialysis technique allows measurement of substances in the extravascular compartment by diffusion. In the described study the microdialysis technique was used to examine the dermal generation of prostaglandin E2 (PGE2) in the skin of isolated perfused bovine udders after topical irritation with arachidonic acid. An increase of the PGE2 concentration in the dialysate factions after topical irritation was measured. The effect was significant 60 minutes after administration of arachidonic acid and rose continuously until the end of the sampling period. Additionally, the microdialysis technique was used to determine the absorption of topically administered compounds. top |
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| August 6, 2000 | Copyright
© 2000 (UNI - Klinikum |
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